Immunoassay Reagents and Kits
Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.
We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.
ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.
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Invitrogen™ Granzyme B Monoclonal Antibody (N4TL33), Brilliant Ultra Violet™ 395, eBioscience™
Mouse Monoclonal Antibody
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| Content And Storage | 4°C, store in dark, DO NOT FREEZE! |
|---|---|
| Target Species | Human |
| Host Species | Mouse |
| Conjugate | Brilliant Ultraviolet 395 |
| Applications | Flow Cytometry |
| Form | Liquid |
| Gene Accession No. | P10144 |
| Isotype | IgG2a κ |
| Concentration | 5 μL/Test |
| Antigen | Granzyme B |
| Gene Symbols | GZMB |
| Regulatory Status | RUO |
| Purification Method | Affinity chromatography |
| Gene Alias | AI553453; C11; Cathepsin G-like 1; CCP1; CCP-1/C11; CCPI; CGL1; CGL-1; CSPB; CSP-B; Ctla1; Ctla-1; CTSGL1; cytotoxic cell protease 1; cytotoxic serine protease B; Cytotoxic T lymphocyte associated serine esterase 1; cytotoxic T-lymphocyte proteinase 2; cytotoxic T-lymphocyte-associated serine esterase 1; fragmentin; fragmentin 2; fragmentin-2; GLP I; GLP III; GLP-1; granzyme 2; granzyme B; granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1); granzyme B(G,H); Granzyme-2; GranzymeB; granzyme-like protein 1; granzyme-like protein I; GRB; GZB; Gzmb; HLP; human lymphocyte protein; Human lymphocyte protein (Hlp); Lymphocyte protease; Natural killer cell protease 1; OTTHUMP00000028189; RNKP-1; SECT; T-cell serine protease 1-3E |
| Gene | GZMB |
| Product Type | Antibody |
| Gene ID (Entrez) | 3002 |
| Formulation | PBS with BSA and 0.09% sodium azide; pH 7.2 |
| Classification | Monoclonal |
| Primary or Secondary | Primary |
| Clone | N4TL33 |
Invitrogen™ Mouse Granzyme B Uncoated ELISA Kit
ELISA
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| Protein Aliases | CTLA-1, Cytotoxic cell protease 1, CCP1, Fragmentin-2 |
|---|---|
| Immunoassay Kit Format | Uncoated ELISA Kit |
| Target Species | Mouse |
| Conjugate | HRP |
| Gene Symbol | GZMB |
| Total Assay Time | 24 hr 30 min |
| Assay Sensitivity | 80 pg/mL |
| Storage Requirements | 2-8°C |
| Assay Range | 80-5000 pg/mL |
| Test Time | 1 hr 45 min |
| Accession Number | P04187 |
| Regulatory Status | RUO |
| Target | Granzyme B |
| Kit Contents | Capture Antibody: Pre-titrated, purified antibody, Detection Antibody: Pre-titrated, biotin-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Enzyme: Pre-titrated Avidin-HRP, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86),Purified antibody,Detection Antibody: Pre-titrated,Biotin-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Enzyme: Pre-titrated Avidin-HRP,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86) |
| Gene Alias | AI553453; C11; Cathepsin G-like 1; CCP1; CCP-1/C11; CCPI; CGL1; CGL-1; CSPB; CSP-B; Ctla1; Ctla-1; CTSGL1; cytotoxic cell protease 1; cytotoxic serine protease B; Cytotoxic T lymphocyte associated serine esterase 1; cytotoxic T-lymphocyte proteinase 2; cytotoxic T-lymphocyte-associated serine esterase 1; fragmentin; fragmentin 2; fragmentin-2; GLP I; GLP III; GLP-1; granzyme 2; granzyme B; granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1); granzyme B(G,H); Granzyme-2; GranzymeB; granzyme-like protein 1; granzyme-like protein I; GRB; GZB; Gzmb; HLP; human lymphocyte protein; Human lymphocyte protein (Hlp); Lymphocyte protease; Natural killer cell protease 1; OTTHUMP00000028189; RNKP-1; SECT; T-cell serine protease 1-3EAI553453; C11; Cathepsin G-like 1; CCP1; CCP-1/C11; CCPI; CGL1; CGL-1; CSPB; CSP-B; Ctla1; Ctla-1; CTSGL1; cytotoxic cell protease 1; cytotoxic serine protease B; Cytotoxic T lymphocyte associated serine esterase 1; cytotoxic T-lymphocyte proteinase 2; cytotoxic T-lymphocyte-associated serine esterase 1; fragmentin; fragmentin 2; fragmentin-2; GLP I; GLP III; GLP-1; granzyme 2; granzyme B; granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1); granzyme B(G,H); Granzyme-2; GranzymeB; granzyme-like protein 1; granzyme-like protein I; GRB; GZB; Gzmb; HLP; human lymphocyte protein; Human lymphocyte protein (Hlp); Lymphocyte protease; Natural killer cell protease 1; OTTHUMP00000028189; RNKP-1; SECT; T-cell serine protease 1-3E; |
| Product Type | ELISA |
| For Use With (Equipment) | Absorbance Microplate Reader |
| Gene ID (Entrez) | 14939 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | Biotin |
Invitrogen™ Mouse MCPT-1 (mMCP-1) Uncoated ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Protein Aliases | mMCP-1 |
|---|---|
| Immunoassay Kit Format | Uncoated ELISA Kit |
| Target Species | Mouse |
| Conjugate | HRP |
| Gene Symbol | Mcpt1 |
| Total Assay Time | 24 hr 30 min |
| Assay Sensitivity | 234 pg/mL |
| Storage Requirements | 2-8°C |
| Assay Range | 234-15000 pg/mL |
| Test Time | 1 hr 45 min |
| Accession Number | P11034 |
| Regulatory Status | RUO |
| Target | MCPT-1 |
| Kit Contents | Capture Antibody: Pre-titrated, purified antibody, Detection Antibody: Pre-titrated, biotin-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, Coating Buffer: 10X PBS ELISA Coating Buffer, Assay Diluent: 5X Concentrated, Detection Enzyme: Pre-titrated Avidin-HRP, Substrate Solution: Tetramethylbenzidine (TMB) Solution, Certificate of Analysis: Lot-specific instructions for the dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22 and -86),Purified antibody,Detection Antibody: Pre-titrated,Biotin-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,Coating Buffer: 10X PBS ELISA Coating Buffer,Assay Diluent: 5X Concentrated,Detection Enzyme: Pre-titrated Avidin-HRP,Substrate Solution: Tetramethylbenzidine (TMB) Solution,Certificate of Analysis: Lot-specific instructions for the dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22 and -86) |
| Gene Alias | AV080368; mast cell protease 1; Mcp-1; Mcpt1; mMCP-1AV080368; mast cell protease 1; Mcp-1; Mcpt1; mMCP-1; IL-12p35; Il-12a; Ll12a; p35;Il-12b; Il-12p40; Il12p40; p40 |
| Product Type | ELISA |
| For Use With (Equipment) | Absorbance Microplate Reader |
| Gene ID (Entrez) | 17224 |
| Sample Type | Serum,Supernatant |
| Label or Dye | Biotin |
Invitrogen™ ProcartaPlex™ Platinum Assay Buffer for Serum Samples
ProcartaPlex Platinum Assay Buffer for Serum Samples (1X) is used for dilution of serum samples and gives spike- and dilution-recovery results in the range of 70 to 130%.
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Invitrogen™ VEGF-A Human ProcartaPlex™ Simplex Kit
The Human VEGF-A Simplex ProcartaPlex Kit measures VEGF-A protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation.
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Invitrogen™ IL-13 Human ProcartaPlex™ Simplex Kit
Ideal solution for assessing multiple protein biomarkers in a single sample. Explore our broad portfolio of pre-configurated panels and combinable single analytes.
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Invitrogen™ SAA Human ProcartaPlex™ Simplex Kit
The Human SAA Simplex ProcartaPlex Kit measures SAA protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation.
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Invitrogen™ Human APRIL ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Target Species | Human |
|---|---|
| Conjugate | HRP |
| Interassay CV | 7.1% |
| Intraassay CV | 8.1% |
| Gene Symbol | TNFSF13 |
| Total Assay Time | 3 hr 10 min |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | 0.4 ng/mL |
| Storage Requirements | 2-8°C |
| Assay Range | 0.78-50.0 ng/mL |
| For Use With (Application) | ELISA |
| Test Time | 1 hr 20 min |
| Accession Number | O75888 |
| Regulatory Status | RUO |
| Target | APRIL |
| Kit Contents | Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Lysis Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Sample Diluent,Assay Buffer concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Lysis Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Absorbance Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 8741 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | Biotin |
| Synonym | TNF 13, TALL 2, TRDL 1, CD256 antigen |
Invitrogen™ Human MMP9 ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Target Species | Human |
|---|---|
| Conjugate | HRP |
| Interassay CV | 10.2% |
| Intraassay CV | 7.3% |
| Gene Symbol | MMP9 |
| Total Assay Time | 3 hr 10 min |
| Sample Volume | Plasma 10 μL, Serum 10 μL, Supernatant 10 μL |
| Assay Sensitivity | 0.05 ng/mL |
| Storage Requirements | 2-8°C |
| Assay Range | 0.23-15.0 ng/mL |
| For Use With (Application) | ELISA |
| Test Time | 1 hr 20 min |
| Accession Number | P14780 |
| Regulatory Status | RUO |
| Target | MMP-9 |
| Kit Contents | Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Controls, Adhesive Plate Covers,Standard,Sample Diluent,Assay Buffer concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Controls,Adhesive Plate Covers |
| For Use With (Equipment) | Absorbance Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 4318 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | Biotin |
| Synonym | 92 kDa gelatinase, Gelatinase B, GELB |
Invitrogen™ Mouse VEGF-A ELISA Kit
ELISA
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| Target Species | Mouse |
|---|---|
| Conjugate | HRP |
| Interassay CV | 6.4% |
| Intraassay CV | 3.8% |
| Gene Symbol | VEGFA |
| Total Assay Time | 4 hr 30 min |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | 20.0 pg/mL |
| Storage Requirements | 2-8°C |
| Assay Range | 62.5-4000 pg/mL |
| For Use With (Application) | ELISA |
| Test Time | 1 hr 20 min |
| Accession Number | Q00731 |
| Regulatory Status | RUO |
| Target | VEGF |
| Kit Contents | Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Sample Diluent,Assay Buffer concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Absorbance Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 22339 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | Biotin |
| Synonym | Vascular endothelial growth factor A, VPF |
Invitrogen™ Rat VEGFC ELISA Kit
ELISA
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| Target Species | Rat |
|---|---|
| Conjugate | HRP |
| Interassay CV | 2.7% |
| Intraassay CV | 5.1% |
| Gene Symbol | VEGFC |
| Total Assay Time | 3 hr 30 min |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | 27.0 pg/mL |
| Storage Requirements | 2-8°C |
| Assay Range | 47.0-3000 pg/mL |
| For Use With (Application) | ELISA |
| Test Time | 1 hr 20 min |
| Accession Number | O35757 |
| Regulatory Status | RUO |
| Target | VEGFC |
| Kit Contents | Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Sample Diluent,Assay Buffer concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Absorbance Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 114111 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | Biotin |
| Synonym | VRP, FLT-4 ligand |
Invitrogen™ Human IL-13 ELISA Kit
ELISA
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| Target Species | Human |
|---|---|
| Conjugate | HRP |
| Interassay CV | 4.6% |
| Intraassay CV | 6% |
| Gene Symbol | Il13 |
| Total Assay Time | 2 hr 10 min |
| Sample Volume | Amniotic Fluid 50 μL, Plasma 50 μL, Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | 0.7 pg/mL |
| Storage Requirements | 2-8°C |
| Assay Range | 1.6-100 pg/mL |
| For Use With (Application) | ELISA |
| Test Time | 1 hr 20 min |
| Accession Number | P35225 |
| Regulatory Status | RUO |
| Target | IL-13 |
| Kit Contents | Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Sample Diluent,Assay Buffer concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Absorbance Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 3596 |
| Sample Type | Amniotic Fluid,Plasma,Serum,Supernatant |
| Label or Dye | Biotin |
| Synonym | ALRH, BHR1 |
Invitrogen™ Mouse IFN gamma ProQuantum Immunoassay Kit
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ELISA
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| Immunoassay Kit Format | Proximity-based Immunoassay |
|---|---|
| Target Species | Mouse |
| Conjugate | FAM |
| Incubation Time | 1 hr. |
| Gene Symbol | IFNG |
| Total Assay Time | 2 hr |
| Sample Volume | Plasma 2-5 μL, Serum 2-5 μL, Supernatant 2-5 μL |
| Assay Sensitivity | <0.05 pg/mL |
| Storage Requirements | -20°C |
| Assay Range | 0.064-5000 pg/mL |
| For Use With (Application) | Protein Quantitation |
| Test Time | 30 min |
| Detection Method | Taqman™ Real-time PCR |
| Accession Number | P01580 |
| Regulatory Status | RUO |
| Target | IFN gamma |
| Kit Contents | Assay Dilution Buffer, Antibody-Conjugate A, Antibody-Conjugate B, Antibody-Conjugate Dilution Buffer, Standard, Master Mix, Ligase,Antibody-Conjugate A,Antibody-Conjugate B,Antibody-Conjugate Dilution Buffer,Standard,Master Mix,Ligase |
| For Use With (Equipment) | qPCR Instruments |
| Product Type | ProQuantum |
| Gene ID (Entrez) | 15978 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | FAM™ |
Invitrogen™ Human IL-18 ProQuantum Immunoassay Kit
ELISA
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| Shipping Condition | Dry Ice |
|---|---|
| Immunoassay Kit Format | Proximity-based Immunoassay |
| Target Species | Human |
| Conjugate | FAM |
| Gene Symbol | IL18 |
| Total Assay Time | 2 hr |
| Assay Sensitivity | <0.05 pg/mL |
| Storage Requirements | -20°C |
| Assay Range | 0.064-1000 pg/mL |
| Test Time | 30 min |
| Accession Number | Q14116 |
| Regulatory Status | RUO |
| Target | IL-18 |
| Kit Contents | Assay Dilution Buffer, Antibody-Conjugate A, Antibody-Conjugate B, Antibody-Conjugate Dilution Buffer, Standard, Master Mix, Ligase,Antibody-Conjugate A,Antibody-Conjugate B,Antibody-Conjugate Dilution Buffer,Standard,Master Mix,Ligase |
| Gene Alias | cytokine; iboctadekin; IFN-gamma-inducing factor; IGIF; il 18; IL-1 gamma; IL18; IL-18; IL1F4; IL-1g; ILN; interferon gamma inducing factor; interferon gamma-inducing factor; interferon-gamma-inducing factor; Interleukin; interleukin 18; interleukin 18 (interferon-gamma-inducing factor); interleukin-1 gamma; Interleukin18; Interleukin-18; interleukin-18 proprotein; interleukin-18-like protein; MGC12320cytokine; iboctadekin; IFN-gamma-inducing factor; IGIF; il 18; IL-1 gamma; IL18; IL-18; IL1F4; IL-1g; ILN; interferon gamma inducing factor; interferon gamma-inducing factor; interferon-gamma-inducing factor; Interleukin; interleukin 18; interleukin 18 (interferon-gamma-inducing factor); interleukin-1 gamma; Interleukin18; Interleukin-18; interleukin-18 proprotein; interleukin-18-like protein; MGC12320; |
| For Use With (Equipment) | qPCR Instruments |
| Product Type | ProQuantum |
| Gene ID (Entrez) | 3606 |
| Sample Type | Plasma,Serum,Supernatant |
Invitrogen™ Mouse IL-2 ELISA Kit
ELISA
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| Target Species | Mouse |
|---|---|
| Conjugate | HRP |
| Interassay CV | 10% |
| Intraassay CV | 5% |
| Gene Symbol | IL2 |
| Total Assay Time | 3 hr 10 min |
| Sample Volume | Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | 5.3 pg/mL |
| Storage Requirements | 2-8°C |
| Assay Range | 15.6-1000 pg/mL |
| For Use With (Application) | ELISA |
| Test Time | 1 hr 20 min |
| Accession Number | P04351 |
| Regulatory Status | RUO |
| Target | IL-2 |
| Kit Contents | Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Sample Diluent,Assay Buffer concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Absorbance Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 16183 |
| Sample Type | Serum,Supernatant |
| Label or Dye | Biotin |
| Synonym | Aldesleukin, T cell growth factor, TCGF |